2011, Vol.14, No.3, pp.295-299
The study of functional state of blood phagocytes adhering to
capacity sensor surface has been carried out by cyclic dielectric
spectroscopy method. The sensor dielectric coating consists of
Langmuir-Blodgett monolayers with multiwall modified carbon
nanotubes. The nonliner dependence of sensor capacity on cell
concentration was registered. At cellular monolayer formation the
Helmholtz double layer electrical field shielding effect was shown
to arise. It has been established that capacity characteristics of
the sensor with adhering monocytes depend on a number and cell
continuity as well as ability of cells to generate reactive oxygen
species. It has been shown that cell activation by adhesion and
fMLP chemotactic peptide leads to increase of capacity of sensor
coated by dense monocyte monolayer.
Key words:
monocyte monolayer, impedance analysis, nanobiosensor,
cell adhesion, monitoring
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